解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Human S100A7 (psoriasin) is a metal-chelating protein expressed by epithelial cells. It is a 22-kDa homodimer with two EF-hand domains per subunit and two transition-metal-binding His3Asp sites at the dimer interface. Each subunit contains two cysteine residues that can exist as free thiols (S100A7red) or as an intram...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00781
更新日期:2017-10-31 00:00:00
abstract::A short chain dehydrogenase like l-threonine 3-dehydrogenase (SDR-TDH) from metagenome data (mtTDH) was identified by database mining. Its enzymatic properties suggested that mtTDH has unique characteristics relative to other SDR-TDHs, including two mesophilic and thermophilic SDR-TDHs identified in this study. The ac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00832
更新日期:2017-10-31 00:00:00
abstract::Histone deacetylase 8 (HDAC8) is a well-characterized member of the class I acetyl-lysine deacetylase (HDAC) family. Previous work has shown that the efficiency of HDAC8-catalyzed deacetylation of a methylcoumarin peptide varies depending on the identity of the divalent metal ion in the HDAC8 active site. Here we demo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00851
更新日期:2017-10-24 00:00:00
abstract::Despite extensive characterization of the human immunodeficiency virus type 1 (HIV-1) hydrophobic fusion peptide (FP), the structure-function relationships underlying its extraordinary degree of conservation remain poorly understood. Specifically, the fact that the tandem repeat of the FLGFLG tripeptide is absolutely ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00745
更新日期:2017-10-17 00:00:00
abstract::Phospholipase Cβ (PLCβ) enzymes hydrolyze phosphatidylinositol 4,5-bisphosphate to produce second messengers that regulate intracellular Ca2+, cell proliferation, and survival. Their activity is dependent upon interfacial activation that occurs upon localization to cell membranes. However, the molecular basis for how ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00547
更新日期:2017-10-17 00:00:00
abstract::Chemical biosensors, for which chemical detection triggers a fluorescent signal, have the potential to accelerate the screening of noncolorimetric chemicals produced by microbes, enabling the high-throughput engineering of enzymes and metabolic pathways. Here, we engineer a G-protein-coupled receptor (GPCR)-based sens...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00605
更新日期:2017-10-17 00:00:00
abstract::Bioluminescence with luciferase-luciferin pairs is an attractive method for surveying cells in live tissues and whole organisms. Recent advances in luciferin chemistry and luciferase engineering are further expanding the scope of the technology. It is now possible to spy on cells in a variety of deep tissues and visua...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.7b00435
更新日期:2017-10-03 00:00:00
abstract::Chemistry, once king of fluorescence microscopy, was usurped by the field of fluorescent proteins. The increased demands of modern microscopy techniques on the "photon budget" require better and brighter fluorophores, causing a renewed interest in synthetic dyes. Here, we review the recent advances in biochemistry, pr...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.7b00529
更新日期:2017-10-03 00:00:00
abstract::The effect of ionic strength on enzyme activity and stability varies considerably between enzymes. Ionic strength is known to affect the catalytic activity of some alkaline phosphatases (APs), such as Escherichia coli AP, but how ions affect APs is debated. Here, we studied the effect of various ions on a cold-adapted...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00690
更新日期:2017-09-26 00:00:00
abstract::By classical competitive antagonism, a substrate and competitive inhibitor must bind mutually exclusively to the active site. The competitive inhibition of O-acetyl serine sulfhydrylase (OASS) by the C-terminus of serine acetyltransferase (SAT) presents a paradox, because the C-terminus of SAT binds to the active site...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00500
更新日期:2017-09-19 00:00:00
abstract::DNA three-way junctions (3WJs) are branched structures that serve as important biological intermediates and as components in DNA nanostructures. We recently derived the global structure of a fully complementary 3WJ and found that it contained unpaired bases at the branchpoint, which is consistent with previous observa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00677
更新日期:2017-09-19 00:00:00
abstract::Anastellin is a small recombinant fragment derived from the extracellular matrix protein fibronectin; it comprises the first type III (FN3) domain without the two N-terminal β-strands. It inhibits angiogenesis, tumor growth, and metastasis in mouse models and requires endogenous fibronectin for its in vivo anti-angiog...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00633
更新日期:2017-09-05 00:00:00
abstract::To study fibronectin (FN) conformation and assembly, we generated several deletion mutants: FNΔI1-5, FNΔIII1-3, FNΔIII4-8, and FNΔIII11-14. A monomeric form, FNmono, which lacked the C-terminal dimerization region, was also created. FNtnA-D was generated by swapping FNIII domains 1-8 in FNΔIII11-14 with seven FNIII do...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00589
更新日期:2017-08-29 00:00:00
abstract::Transporters from the SLC13 family couple the transport of two to four Na+ ions with a di- or tricarboxylate, such as succinate or citrate. We have previously modeled mammalian members of the SLC13 family, including the Na+/dicarboxylate cotransporter NaDC1 (SLC13A2), based on a structure of the bacterial homologue Vc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00503
更新日期:2017-08-22 00:00:00
abstract::The Plasmodium falciparum malarial parasite genome appears to encode one and only one phosphatidylinositol 3'-kinase (PI3K), and sequence analysis suggests that the enzyme is a "class III"- or "Vps34"-type PI3K. PfVps34 has generated excitement as a possible druggable target and potentially a key target of artemisinin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00416
更新日期:2017-08-22 00:00:00
abstract::We present a study of the interactions between the lipase from Thermomyces lanuginosus (TlL) and the two microbially produced biosurfactants (BSs), rhamnolipid (RL) and sophorolipid (SL). Both RL and SL are glycolipids; however, RL is anionic, while SL is a mixture of anionic and non-ionic species. We investigate the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00420
更新日期:2017-08-15 00:00:00
abstract::We previously showed that a bulky ring-strained cycloalkyne possessing a rhodamine fluorophore directly reacts (via strain-promoted click chemistry) with the azido group incorporated (via ligand-directed tosyl chemistry) into Asp160 in the 49 kDa subunit of complex I in bovine heart submitochondrial particles [Masuya,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00612
更新日期:2017-08-15 00:00:00
abstract::l-Aspartate oxidase, encoded by the nadB gene, is the first enzyme in the de novo synthesis of NAD+ in bacteria. This FAD-dependent enzyme catalyzes the oxidation of l-aspartate to generate iminoaspartate and reduced flavin. Distinct from most amino acid oxidases, it can use either molecular oxygen or fumarate to reox...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00307
更新日期:2017-08-08 00:00:00
abstract::Our previous studies demonstrated that the first 1000 amino acid residues (the βα1 domain) of human apolipoprotein (apo) B-100, termed apoB:1000, are required for the initiation of lipoprotein assembly and the formation of a monodisperse stable phospholipid (PL)-rich particle. The objectives of this study were (a) to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01174
更新日期:2017-08-08 00:00:00
abstract::Intrinsically disordered proteins (IDPs) are functionally active despite lacking a well-defined three-dimensional structure. Such proteins often undergo a disorder-to-order transition, or induced folding, when binding to their specific physiological partner. Because of cooperativity, the folding and binding steps typi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00350
更新日期:2017-07-25 00:00:00
abstract::During catalysis by liver alcohol dehydrogenase (ADH), a water bound to the catalytic zinc is replaced by the oxygen of the substrates. The mechanism might involve a pentacoordinated zinc or a double-displacement reaction with participation by a nearby glutamate residue, as suggested by studies of human ADH3, yeast AD...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00446
更新日期:2017-07-18 00:00:00
abstract::RNA repeat expansions cause a host of incurable, genetically defined diseases. The most common class of RNA repeats consists of trinucleotide repeats. These long, repeating transcripts fold into hairpins containing 1 × 1 internal loops that can mediate disease via a variety of mechanism(s) in which RNA is the central ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00252
更新日期:2017-07-11 00:00:00
abstract::Halogen bonding is a weak chemical force that has so far mostly found applications in crystal engineering. Despite its potential for use in drug discovery, as a new molecular tool in the direction of molecular recognition events, it has rarely been assessed in biopolymers. Motivated by this fact, we have developed a p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00429
更新日期:2017-06-27 00:00:00
abstract::BamA is an essential component of the β-barrel assembly machine (BAM) that is responsible for insertion and folding of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. BamA is an OMP itself, and its β-barrel transmembrane domain is thought to catalyze OMP insertion and folding, although the molecular...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00281
更新日期:2017-06-20 00:00:00
abstract::Nonenzymatic deamidation of asparagine and glutamine in peptides and proteins is a frequent modification both in vivo and in vitro. The biological effect is not completely understood, but it is often associated with protein degradation and loss of biological function. Here we describe the deamidation of CsgA, the majo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00241
更新日期:2017-06-13 00:00:00
abstract::Oxalate:ferredoxin oxidoreductase (OOR) is an unusual member of the thiamine pyrophosphate (TPP)-dependent 2-oxoacid:ferredoxin oxidoreductase (OFOR) family in that it catalyzes the coenzyme A (CoA)-independent conversion of oxalate into 2 equivalents of carbon dioxide. This reaction is surprising because binding of C...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00222
更新日期:2017-06-06 00:00:00
abstract::As a prerequisite to mammalian fertilization, the sperm acrosomal vesicle fuses with the plasma membrane and the acrosome contents are exocytosed. Induction occurs through engagement of the sperm receptors by multiple sugar residues. Multivalent polymers displaying mannose, fucose, or GlcNAc are effective synthetic in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00166
更新日期:2017-06-06 00:00:00
abstract::In mammalian cells, the incorporation of the 21st amino acid, selenocysteine, into proteins is guided by the Sec machinery. The function of this protein complex requires several protein-protein and protein-RNA interactions, leading to the incorporation of selenocysteine at UGA codons. It is guided by stem-loop structu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01116
更新日期:2017-05-02 00:00:00
abstract::The photoautotrophic freshwater cyanobacterium Synechococcus elongatus is widely used as a chassis for biotechnological applications as well as a photosynthetic bacterial model. In this study, a method for expanding the genetic code of this cyanobacterium has been established, thereby allowing the incorporation of unn...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00131
更新日期:2017-04-25 00:00:00
abstract::Prolyl oligopeptidase B from Galerina marginata (GmPOPB) has recently been discovered as a peptidase capable of breaking and forming peptide bonds to yield a cyclic peptide. Despite the relevance of prolyl oligopeptidases in human biology and disease, a kinetic analysis pinpointing rate-limiting steps for a member of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00012
更新日期:2017-04-18 00:00:00
abstract::Z-disc-associated, alternatively spliced, PDZ motif-containing protein (ZASP) is a principal component of the sarcomere. The three prevalent isoforms of ZASP in skeletal muscle are generated by alternative splicing of exons 9 and 10. The long isoforms, either having (ZASP-L) or lacking exon 10 (ZASP-LΔex10), include a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00067
更新日期:2017-04-11 00:00:00
abstract::Histone tails in nucleosomes play critical roles in regulation of many biological processes, including chromatin compaction, transcription, and DNA repair. Moreover, post-translational modifications, notably lysine acetylation, are crucial to these functions. While the tails have been intensively studied, how the stru...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01208
更新日期:2017-04-11 00:00:00
abstract::Heme (iron protoporphyrin IX) is an essential protein prosthetic group and signaling molecule required for most life on Earth. All heme-dependent processes require the dynamic and rapid mobilization of heme from sites of synthesis or uptake to hemoproteins present in virtually every subcellular compartment. The cytoto...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00007
更新日期:2017-04-04 00:00:00
abstract::Designing subtype-selective agonists for neuronal nicotinic acetylcholine receptors is a challenging and significant goal aided by intricate knowledge of each subtype's binding patterns. We previously reported that in α6β2 receptors, acetylcholine makes a functional cation-π interaction with Trp149, but nicotine and T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00213
更新日期:2017-04-04 00:00:00
abstract::A major biochemical goal is the ability to mimic nature in engineering highly specific protein-protein interactions (PPIs). We previously devised a computational interactome screen to identify eight peptides that form four heterospecific dimers despite 32 potential off-targets. To expand the speed and utility of our a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00047
更新日期:2017-03-21 00:00:00
abstract::In the following, molecular simulations are used to reveal unexpected behavior within bacterial membranes. We show that lipopolysaccharide molecules found in these membranes form viscous amorphous solids when they are interlinked with monovalent and divalent cations. The bilayers exhibit both liquid and glassy charact...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01063
更新日期:2017-03-21 00:00:00
abstract::Cuprizone intoxication is a common animal model used to test myelin regenerative therapies for the treatment of diseases such as multiple sclerosis. Mice fed this copper chelator develop reversible, region-specific oligodendrocyte loss and demyelination. While the cellular changes influencing the demyelinating process...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01072
更新日期:2017-03-14 00:00:00
abstract::βγ-Crystallins are important constituents of the vertebrate eye lens, whereas in microbes, they are prevalent as Ca2+-binding proteins. In archaea, βγ-crystallins are conspicuously confined to two methanogens, viz., Methanosaeta and Methanosarcina. One of these, i.e., M-crystallin from Methanosarcina acetivorans, has ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00985
更新日期:2017-03-07 00:00:00
abstract::In synthetic biology, metabolic engineering, and gene therapy, there is a strong demand for orthogonal or externally controlled regulation of gene expression. Here, RNA-based regulatory devices represent a promising emerging alternative to proteins, allowing a fast and direct control of gene expression, as no synthesi...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.6b01218
更新日期:2017-03-07 00:00:00
abstract::Protein splicing is a post-translational reaction facilitated by an intein, or intervening protein, which involves the removal of the intein and the ligation of the flanking polypeptides, or exteins. A DNA polymerase II intein from Pyrococcus abyssi (Pab PolII intein) can promote protein splicing in vitro on incubatio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00894
更新日期:2017-02-28 00:00:00